Electrophysiology

Your channel, our solution

Are you working with electrogenic proteins expressed on plasma or organellar membranes? Do you need support studying ion channel, transporter or exchanger biology? Our team of skilled electrophysiologists have at their disposal an eclectic panel of both manual and automated patch-clamp devices allowing them to define the best strategy to assist your request.

During the assay development phase, our platforms allow us to pharmacologically and biophysically validate “in house
generated” or transferred cell lines. Then, using the most suitable tools, we design fine-tuned experimental conditions to perform small and medium-scale compound testing and primary screenings, as well as hit validation experiments and mode of action studies on ligand- and voltage-gated ion channels using the proper configuration (cell attached, whole-cell, inside-out or outside-out).

Discover our patch clamp platforms

Whole cell or single channel (inside- and outside-out configurations) recordings
Cellular and organellar electrophysiology
Low throughput compound profiling & mode-of-action studies
Optogenetics
iPSC-derived cell electrophysiology (temperature control)
Fast internal (Port-a-Patch) and external solution exchange

Medium-high throughput screening for hit discovery, hit confirmation and SAR campaign
On off-the-shelf assays or adapted clients’ assays
Preliminary cardiac safety profiling
Suitable for iPSC-derived cell electrophysiology
Current clamp and temperature control available
Implemented for Genedata analysis settings

Fully automated high throughput screening for hit discovery, hit confirmation and SAR campaign
On tailored-made assays or adapted clients’ assays
Suitable for clone selection during cell line generation
Fast internal solution exchange
iPSC-derived cell electrophysiology
Current clamp and temperature control available
Optogenetics (opto-SyncroPatch)
Implemented for Genedata analysis settings
Automated LysoPatch (worldwide premiere)

Off-the-shelf assays

Axxam has already validated several assays that can immediately been used for services and additional assays can be adapt upon client’s request.

Interested in going deeper into the cell?

In the last few years, we have developed a huge expertise on organellar electrophysiology enabling the study of ion channels localized on the membrane of nuclei (manual NucleoPatch), lysosomes (LysoPatch and automated LysoPatch) and mitochondria (manual MitoPatch).