Webinars

July 8th, 2015: Optogenetics and Cellular Assays: The revolutionary use of light to drive High Throughput Screening

The Webinar will address the following topics:

Light activated proteins have been used to optically modulate biophysic or metabolic pathways of complex organisms with high spatial and temporal precision, providing novel and valuable alternatives to existing electrical or chemical perturbation techniques. AXXAM has now transferred the advantages of optogenetics to High Throughput Screening by validating a panel of “light actuator” and “light sensor” tools for the study of different cellular targets:

  • Channelrhodopsin-2 to modulate voltage gated calcium channel activity and study state-dependent blockers
  • bPAC enzyme to study HCN2 ion channel activity
  • ArcLight voltage sensor to detect Kir, NaV, TMEM or ASIC ion channel activity
  • RCaMP calcium sensor to detect muscarinic receptor activity

If you missed the webinar and would like to receive information about this topic, please send a request at this link


February 26th, 2015: Beyond the Microbiome- A screening platform targeting metabolic disease

The Webinar addressed  the following topics:
- Introduction to the in-vitro platform  developed at Axxam for GPCRs involved in metabolism
- cell-based assays for GLP1 secretion
- cell lines stably expressing the chemosensors: GPR40, GPR43, GPR93, GPR119, GPR120, and  GPR131
- cell lines expressing bitter receptors found in the GI tract
- enzymatic and cell-based assay for GOAT

If you missed the webinar and would like to receive information about this topic, please send a request at this link


October 21st , 2014 : The Bitter Truth – Modulating Taste by Targeting Bitter Receptors 

The webinar addressed the following topics:
- Introduction to bitter taste receptors and their role in gustatory functions
- Development of the bitter taste receptor platform 
- Insight into the pharmacological characterization of bitter taste receptors
- Application of the bitter taste receptor platform in food & beverage industries
- Case study on the identification of a bitter masking compound

If you missed the webinar and would like to receive information about this topic, please send a request at this link


September 16th, 2014: Gene-Editing Nucleases: Cutting-edge Tools for a Next Generation of Cell-Based Assays

Gene-editing nucleases (Nature Methods 2011 Method of the Year) are able to introduce targeted, tailored changes into the genome of several species and are powerful tools for mediating genome alteration with high precision. A number of gene editing technologies have emerged in the past years: AXXAM focuses on the use of the most recent TALEN and CRISPR/Cas9 nuclease to modify genes of interest in a highly site-specific way by inserting, deleting or editing endogenous genomic DNA sequences, ideally leaving an isogenic background in the cell.  Case studies using both technologies will be presented to evaluate the advantages of the different

If you missed the webinar and would like to receive information about this topic, please send a request at this link



May 6th and 8th, 2014 : Functional Bitter Taste Receptor platform and its application for taste modulation, airway biology and gastro-intestinal systems

Bitter compounds of different natural or artificial origins are detected by a specific subset of taste receptor cells localized in the mouth and characterized by the expression of the TAS2R gene family encoding bitter taste receptors. Peripheral expression in airways and the gastro-intestinal tract has been reported suggesting important non-gustatory functions of bitter taste receptors.
At Axxam, we have established a cellular platform encompassing all 25 human bitter taste receptors and variants thereof.
This platform of functional assays represents a valuable tool which allows us to match any bitter compound with its respective
receptor and to subsequently identify receptor modulators.

If you missed the webinar and would like to receive information about this topic, please send a request at this link



18th and 20th March 2014: Optogenetics: a bright future for voltage Gated Ion Channels

Optogenetic is a technology that combines  genetic and optical methods to achieve fast control of well-defined events in specific cells of living tissue.The technology is based on the use of Opsins,  a family of seven-transmembrane, light-responsive proteins, that rapidly translocate specific ions across the membranes of the cells in which they are expressed.

At Axxam laboratories, a proof of principle study has been performed to verify the applicability of this tool to the development of cell based assays suitable for running High Throughput Screening (HTS).
This webinar will address the following topics:

•              Introduction to Optogenetics technology
•              Development of cell lines
•              Application  of the cell line to HTS instrumentation
•              Future perspectives and application of the technology

If you missed the webinar and would like to receive copy of the presentation please send a request at this link