Axxam provides a comprehensive and detailed Hits to Leads service to maximize the results from your primary screening campaign. We have an extensive range of orthogonal assay formats that can be applied to weed out the false positive results, and confirm the real hits. We apply our in-depth knowledge of target biology and small molecule chemical analysis to define the most promising structures and scaffolds to take forward in your Lead Optimization and ADMET programs.

Our Hits to Leads capabilities cover a wide range of activities and for any given project may include:

• Hit Confirmation - hits are rigorously re-screened and analyzed to confirm their authenticity against the primary target
• Re-testing - all hits are re-run with the same assay used for primary screening
• Orthogonal testing - hits re-run in different assay formats
• Secondary screening - more rigorous conditions applied in further functional tests
• Dose-response curve generation - IC₅₀ and EC₅₀ curves established to determine relative potencies
• General Counter screen - false positives are eliminated using a similar assay against another target or the parental cell line screen for a cell based assay
• Data analysis - extensive data analysis capabilities utilizing our custom Spotfire^® and Genedata^® analysis programs
• Chemical relevance - utilizing in-silico structural analysis and our in-house medicinal chemistry resource we work with our clients to identify the most promising molecules
• Novelty & patentability - our in-house IP legal resources and medicinal chemistry staff work closely to recommend the potentially most valuable scaffolds and structures to take forward to Lead Optimization

Please, contact us to find our more about our Hits to Leads capabilities and offering.