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Quality and flexibility underpin the Axxam approach to cell-based assays for high-throughput screening. Choosing Axxam for your cell-based assay requirements means more than a ‘list’ of assays to select from. Much more than a vial of frozen cells.
We know that successful hits from HTS campaigns are most likely to come from functional assays. That’s why at Axxam we have concentrated on building a core competency in generating functional cell-based assays. Indeed, this core competency is was established years ago when we were part of the worldwide R&D organization of Bayer AG with specialized and dedicated laboratories for the development of specific ultra high-throughput screening assays.
Axxam cell biologists have developed a comprehensive platform of cell-based assays suitable for HTS for all classes
of druggable and assayable targets. By using reporter genes and tools, our cell biologists adhere to an intensive process
to develop functional cell-based assays to study the activation of the target of interest.
Detection systems include both fluorescence and luminescence with assays optimized for standard 96-, 384- and 1536-MTP formats. Available functional assays can be further divided according to the technology platform utilized to detect the type of induced signaling upon target activation – glow or flash luminescence and FLIPR® technology.
This competency extends to R&D at Axxam also. We’ve developed Photina® – a new and improved photoprotein to improve the quality and sensitivity of Ca2+ activated assays.
If, by chance, we have not yet developed assays for the target you want to investigate, we collaborate closely with your researchers to establish a detailed development plan for the assay. Where possible more than one experimental approach is followed and our aim is to find the most sensitive system to satisfy your assay requirements.
The assay configured and optimized at Axxam is finally transferred to your laboratories accompanied by one of our scientists, who will spend a full working week transferring and adapting the assay to your facilities. |
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